Protein Purification Reagents and Kits
STEMCELL Technologies EasySep™ Mouse APC Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Mouse APC Positive Selection Kit II is designed to isolate cells that are labeled with APC (allophycocyanin)-conjugated antibodies. Desired cells are targeted with antibody complexes recognizing APC and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Mouse APC Positive Selection Kit (Catalog #18452).
Norgen Biotek Corp Saliva Exosome Collection and Preservation Kit
Norgen’s Saliva Exosome Collection and Preservation Kit is an all-in-one solution designed for 1) simple and non-invasive saliva collection; 2) preservation of exosomes in saliva samples at ambient temperature; and 3) isolation of high quality intact exosomes and exosomal RNA in a laboratory setting. The Saliva Exosome Collection, Preservation and Isolation Kit contains 50 Individual Saliva Exosome Collection and Preservation Devices, as well as the required reagents for the subsequent laboratory purification of intact exosomes and isolation of the exosomal RNA from the preserved samples. Each of the 50 individual Saliva Exosome Collection and Preservation Devices consists of 3 components: (1) Saliva Collection Funnel and Collection Tube that contains Norgen’s Urine Preservative in a dried format, (2) Collection Tube Cap, and (3) ID Label. The saliva exosomes in preserved samples is stable for 2 years at room temperature.
STEMCELL Technologies EasySep™ Human Memory B Cell Isolation Kit, For processing: 2 x 10^9 cells
The EasySep™ Human Memory B Cell Isolation Kit is a two-step method designed to isolate CD27+ B cells from fresh or previously frozen peripheral blood mononuclear cells. First, CD27+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD27+ cells, and unwanted non-B cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified CD27+ memory B cells that are immediately ready for downstream applications. An optional protocol allows for the isolation of naïve B cells in parallel for use in functional studies.
Abcam His-Tag Protein Purification Column (Pre-packed, 5 x 5ml) - AminTrap, 5UNIT
His-Tag Protein Purification Column (Pre-packed, 5 x 5ml) - AminTrap (ab270529) is a simple and easy ready to use chromatography medium for purifying His-tagged proteins that have been expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells.
The product is subject to the following: Abcam Restricted Use Statement
Genscript Corporation GST Fusion Protein Purification Kit
Glutathione Resin (Cat. No. L00206) is an affinity chromatography medium designed for easy, one-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in E. coli, insect cells and mammalian cells. The recombinant GST fusion proteins can be purified directly from pre-treated cell lysate using Glutathione Resin. It is the excellent choice for high performance purifications.
STEMCELL Technologies EasySep™ Human Th17 Cell Enrichment Kit II, For processing: 2 x 10^9 cells
The EasySep™ Human Th17 Enrichment Kit II is a two-step method designed to enrich for human Th17 cells from fresh peripheral blood mononuclear cells. First, CCR6+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the cells and unwanted non-CD4+ T cells, CD45RA+ cells, and CXCR3+ cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction is enriched for IL-17-producing Th17 cells.This product can be used in place of the EasySep™ Human Th17 Cell Enrichment Kit (Catalog #18162) for even faster cell isolations.
Norgen Biotek Corp Cytoplasmic & Nuclear RNA Purification Kit, 50 Prep
This kit provides a rapid method for the isolation and purification of both cytoplasmic and nuclear RNA from cultured animal cells and small tissue samples. The kit can be used to isolate all sizes of RNA from the cytoplasmic and nuclear RNA fractions, including all small RNA species without any requirement for phenol. Included in the kit are sufficient reagents to perform either 50 cytoplasmic RNA preparations or 25 cytoplasmic and 25 nuclear RNA preparations. Ten samples can be processed in approximately 45 minutes. This kit is also available in a 100 prep size.
STEMCELL Technologies EasySep™ Human CD19 Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human CD19 Positive Selection Kit II is designed to isolate CD19+ cells from fresh or previously frozen peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD19 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD19 antigen is expressed on mature B cells and B cell progenitors but is lost upon maturation to plasma cells. It is also expressed on follicular dendritic cells.This product replaces the EasySep™ Human CD19 Positive Selection Kit (Catalog #18054) for even faster cell isolations.
STEMCELL Technologies RoboSep™ Human CD14 Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human CD14 Positive Selection Kit II is designed to isolate highly purified CD14+ cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs). Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD14 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD14 antigen is strongly expressed on monocytes and macrophages and weakly on granulocytes. It is also expressed on most tissue macrophages.This product replaces the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058), for even faster cell isolations and is compatible with all EasySep™ magnets.
STEMCELL Technologies RoboSep™ Human Monocyte Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Monocyte Isolation Kit is designed to isolate CD14+CD16- monocytes from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells and platelets with antibody complexes and magnetic particles. The magnetically labelled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.This product can be used in place of the EasySep™ Human Monocyte Enrichment Kit (Catalog #19059) for even faster cell isolations with reduced platelet contamination.
Enzo Life Sciences EPIXTRACT Nuclear Protein Isolation Kit II (1x100Reactions)
Rapid, reliable method to isolate nucleic acid-free nuclear proteins in mammalian cells and tissues. EPIXTRACT Nuclear Protein Isolation Kit II provides a simple and reliable method to isolate nucleic acid-free nuclear proteins. Extracts can be used for a variety of downstream applications including western blotting, protein-DNA binding assays, nuclear enzyme assays and others requiring nucleic acid-free nuclear proteins. Assay Time: 1 hour. Contents: Pre-extraction Buffer, Extraction Buffer, Extraction pre-cleaner, Extraction cleaner, Protease Inhibitor Cocktail, DTT
Norgen Biotek Corp RNA/DNA/Protein Purification Plus Kit, 50 preps, spin-column method, processes in < 30 min, compatible with cultured animal cells, tissues, blood, yeast, bacteria, fungi, plants
The Norgen Biotek Corp RNA/DNA/Protein Purification Plus Kit offers a rapid and efficient spin-column method for the isolation and purification of total RNA, genomic DNA, and proteins from a single biological sample. This kit is designed for researchers studying various biological topics while ensuring reliable analysis from precious or small samples. The purification process can be completed in less than 30 minutes, yielding high-integrity macromolecules suitable for a wide range of downstream applications such as real-time PCR, sequencing, and Western blots.
- Sequential purification of total RNA, genomic DNA, and proteins from a single sample
- Rapid processing completed in under 30 minutes
- Compatible with numerous sample types, including cultured animal cells, blood, and plants
- Produces high-purity RNA, DNA, and proteins ready for downstream applications
- Comprehensive recovery of RNA sizes including microRNA
CUBE BIOTECH PureCube 100 Ni-NTA Agarose; 50 ml
50 ml of Ni-INDIGO MagBeads for His-tag-purification, 50% slurry
Cederlane LYMPHOLYTE-RAB CELL SEP 500ML
Lympholyte-Rabbit Cell Separation Media (Isolation of lymphocytes from Rabbit lymphoid tissue), liquid 500ml